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Other alkaloids, such as nuciferine (from lotus, Nelumbo nucifera ), likewise inhibit inflammatory signaling and oxidative stress in vascular cells, contributing to anti-atherosclerotic effects (Xiao et al., 2023)

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The increased endothelial cell permeability is generated by TGF1 via SMAD3-dependent signaling, which upregulates matrix metalloproteinase-9 and downregulates claudin-5

You may need to restart at a lower dose to avoid significant gastrointestinal side effects as your body readjusts to the medication

and Bkdal et al

Additionally, during the acridine orange (AO) reaction for a lysosome membrane permeability assay, the cells with indicated treatments were stained with 2 g/mL AO (Invitrogen) for 15 min at 37 C