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After centrifugation (15 min, 12,000 g, 4 C), the upper aqueous phase was carefully collected and transferred into a new tube containing 1 ml of freshly prepared 70% ethanol solution, mixed by pipetting, and loaded onto a RNeasy spin column (RNeasy mini kit, Qiagen)

Reconstitution by the researcher also allows control over the final solution concentration

Think of it like this: a doctor needs an X-ray to confirm a broken bone before putting on a cast

For mild deficiencies or preventative supplementation, oral pills may suffice

To be reconstituted (see instructional video)

Consult your healthcare provider for personalized advice