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After spinning, the filter disks and protein precipitation plate were discarded, and the eluate was immediately used for biomarker analysis in singlecates by Simoa technology on the HD-X platform using a neat protocol (ALZpath Simoa pTau-217 v2 Assay 3 (Quanterix, catalogue number 104371), Simoa Neurology 4-plex E (Quanterix, catalogue number 103607) or Simoa Neurology 2-plex B (Quanterix, catalogue number 103520)

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For treatment over several days or in mild cases, 300 to 600 mg per day should be injected into a muscle or continuously injected into a vein