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After microscopic examination, oocytes at metaphase II stages (MII, with the polar body extrusion) were collected and cultured in 500 l of fertilization droplets as SOF medium supplemented with 5% fetal bovine serum, 2 mM sodium pyruvate, 10 g/ml heparin sodium and 1 mM caffeine in a CO 2 incubator (38.5C, 90%N 2 , 5%CO 2 and 5%O 2 ) (32)

Gently agitate the contents to mix the solution

I 2 = 0%), or hypoglycemia in patients with T2DM (OR: 1.06, 95% CI: 0.37, 3.07

0.52.5 min, 95% B

This product should be kept in the original bottle, tightly closed with the original desiccant remaining in the bottle.

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