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Working With the BPC-157 Peptide in Research Settings Researchers working with this compound should be familiar with several practical considerations that affect the quality and reproducibility of experimental results

Measurement of reactive oxygen species and lipid peroxidation Cells were incubated with 5 M BODIPY 581/591 C11 (Life technologies) for lipid peroxidation, or 5 M CM-H2DCFDA (Life technologies) for ROS in the dark for 30 minutes at 37 C

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Standard reconstitution uses 5ml bacteriostatic water for a 50mg vial, producing 10mg/ml concentration

doi: 10.1136/annrheumdis-2012-201526 294 KarouzakisERazaKKollingCBuckleyCDGaySFilerAet al

Batg P, Barczyski B, Ruszaa M, Paluszkiewicz P