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When they settled down to sleep that night

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Next, the membranes were incubated with Alexa Fluor 488 secondary antibody in PBS-C/M containing 16.2 M Hoechst 33342 (Thermo Fisher Scientific) for 30 min at room temperature

Use guidance to establish norms or soft standards that can be used to expedite applications if met, but require further substantiation and investigation if those standards are not met

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